Partnership building is one of the thrusts of the TDF to implement its mission and attain its vision. In the spirit of private public partnership, it has partnered with the Department of Health.

Its international partners include the World Health Organization, the International Union against TB and Lung Diseases, the Centers for Disease Control and Prevention, USA, the TB Research Unit of the Case Western Reserve University in Cincinnati, Ohio, and the Foundation for Innovative New Diagnostics. The TDF is a member organization of the Working Group on MDR-TB, the STOP TB Partnership, and the Global Alliance for TB Drug Development.

National partners include the Philippine Coalition against Tuberculosis of which it is one of the founding members, the World Vision Development Foundation, theNutrition Center of the Philippines, and the Philippine NGO Council for Population and Health and Welfare.